how to introduce hypothesis in research paper sample

Writing an Introduction for a Scientific Paper

Dr. michelle harris, dr. janet batzli, biocore.

This section provides guidelines on how to construct a solid introduction to a scientific paper including background information, study question , biological rationale, hypothesis , and general approach . If the Introduction is done well, there should be no question in the reader’s mind why and on what basis you have posed a specific hypothesis.

Broad Question : based on an initial observation (e.g., “I see a lot of guppies close to the shore. Do guppies like living in shallow water?”). This observation of the natural world may inspire you to investigate background literature or your observation could be based on previous research by others or your own pilot study. Broad questions are not always included in your written text, but are essential for establishing the direction of your research.

Background Information : key issues, concepts, terminology, and definitions needed to understand the biological rationale for the experiment. It often includes a summary of findings from previous, relevant studies. Remember to cite references, be concise, and only include relevant information given your audience and your experimental design. Concisely summarized background information leads to the identification of specific scientific knowledge gaps that still exist. (e.g., “No studies to date have examined whether guppies do indeed spend more time in shallow water.”)

Testable Question : these questions are much more focused than the initial broad question, are specific to the knowledge gap identified, and can be addressed with data. (e.g., “Do guppies spend different amounts of time in water <1 meter deep as compared to their time in water that is >1 meter deep?”)

Biological Rationale : describes the purpose of your experiment distilling what is known and what is not known that defines the knowledge gap that you are addressing. The “BR” provides the logic for your hypothesis and experimental approach, describing the biological mechanism and assumptions that explain why your hypothesis should be true.

The biological rationale is based on your interpretation of the scientific literature, your personal observations, and the underlying assumptions you are making about how you think the system works. If you have written your biological rationale, your reader should see your hypothesis in your introduction section and say to themselves, “Of course, this hypothesis seems very logical based on the rationale presented.”

A thorough rationale defines your assumptions about the system that have not been revealed in scientific literature or from previous systematic observation. These assumptions drive the direction of your specific hypothesis or general predictions.
Defining the rationale is probably the most critical task for a writer, as it tells your reader why your research is biologically meaningful. It may help to think about the rationale as an answer to the questions— how is this investigation related to what we know, what assumptions am I making about what we don’t yet know, AND how will this experiment add to our knowledge? *There may or may not be broader implications for your study; be careful not to overstate these (see note on social justifications below).
Expect to spend time and mental effort on this. You may have to do considerable digging into the scientific literature to define how your experiment fits into what is already known and why it is relevant to pursue.
Be open to the possibility that as you work with and think about your data, you may develop a deeper, more accurate understanding of the experimental system. You may find the original rationale needs to be revised to reflect your new, more sophisticated understanding.
As you progress through Biocore and upper level biology courses, your rationale should become more focused and matched with the level of study e ., cellular, biochemical, or physiological mechanisms that underlie the rationale. Achieving this type of understanding takes effort, but it will lead to better communication of your science.

***Special note on avoiding social justifications: You should not overemphasize the relevance of your experiment and the possible connections to large-scale processes. Be realistic and logical —do not overgeneralize or state grand implications that are not sensible given the structure of your experimental system. Not all science is easily applied to improving the human condition. Performing an investigation just for the sake of adding to our scientific knowledge (“pure or basic science”) is just as important as applied science. In fact, basic science often provides the foundation for applied studies.

Hypothesis / Predictions : specific prediction(s) that you will test during your experiment. For manipulative experiments, the hypothesis should include the independent variable (what you manipulate), the dependent variable(s) (what you measure), the organism or system , the direction of your results, and comparison to be made.

If you are doing a systematic observation , your hypothesis presents a variable or set of variables that you predict are important for helping you characterize the system as a whole, or predict differences between components/areas of the system that help you explain how the system functions or changes over time.

Experimental Approach : Briefly gives the reader a general sense of the experiment, the type of data it will yield, and the kind of conclusions you expect to obtain from the data. Do not confuse the experimental approach with the experimental protocol . The experimental protocol consists of the detailed step-by-step procedures and techniques used during the experiment that are to be reported in the Methods and Materials section.

Some Final Tips on Writing an Introduction

As you progress through the Biocore sequence, for instance, from organismal level of Biocore 301/302 to the cellular level in Biocore 303/304, we expect the contents of your “Introduction” paragraphs to reflect the level of your coursework and previous writing experience. For example, in Biocore 304 (Cell Biology Lab) biological rationale should draw upon assumptions we are making about cellular and biochemical processes.
Be Concise yet Specific: Remember to be concise and only include relevant information given your audience and your experimental design. As you write, keep asking, “Is this necessary information or is this irrelevant detail?” For example, if you are writing a paper claiming that a certain compound is a competitive inhibitor to the enzyme alkaline phosphatase and acts by binding to the active site, you need to explain (briefly) Michaelis-Menton kinetics and the meaning and significance of Km and Vmax. This explanation is not necessary if you are reporting the dependence of enzyme activity on pH because you do not need to measure Km and Vmax to get an estimate of enzyme activity.
Another example: if you are writing a paper reporting an increase in Daphnia magna heart rate upon exposure to caffeine you need not describe the reproductive cycle of magna unless it is germane to your results and discussion. Be specific and concrete, especially when making introductory or summary statements.

Where Do You Discuss Pilot Studies? Many times it is important to do pilot studies to help you get familiar with your experimental system or to improve your experimental design. If your pilot study influences your biological rationale or hypothesis, you need to describe it in your Introduction. If your pilot study simply informs the logistics or techniques, but does not influence your rationale, then the description of your pilot study belongs in the Materials and Methods section.

How will introductions be evaluated? The following is part of the rubric we will be using to evaluate your papers.

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Step-by-Step Guide: How to Craft a Strong Research Hypothesis

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Table of Contents

A research hypothesis is a concise statement about the expected result of an experiment or project. In many ways, a research hypothesis represents the starting point for a scientific endeavor, as it establishes a tentative assumption that is eventually substantiated or falsified, ultimately improving our certainty about the subject investigated.

To help you with this and ease the process, in this article, we discuss the purpose of research hypotheses and list the most essential qualities of a compelling hypothesis. Let’s find out!

How to Craft a Research Hypothesis

Crafting a research hypothesis begins with a comprehensive literature review to identify a knowledge gap in your field. Once you find a question or problem, come up with a possible answer or explanation, which becomes your hypothesis. Now think about the specific methods of experimentation that can prove or disprove the hypothesis, which ultimately lead to the results of the study.

Enlisted below are some standard formats in which you can formulate a hypothesis¹ :

A hypothesis can use the if/then format when it seeks to explore the correlation between two variables in a study primarily.

Example: If administered drug X, then patients will experience reduced fatigue from cancer treatment.

A hypothesis can adopt when X/then Y format when it primarily aims to expose a connection between two variables

Example: When workers spend a significant portion of their waking hours in sedentary work , then they experience a greater frequency of digestive problems.

A hypothesis can also take the form of a direct statement.

Example: Drug X and drug Y reduce the risk of cognitive decline through the same chemical pathways

What are the Features of an Effective Hypothesis?

Hypotheses in research need to satisfy specific criteria to be considered scientifically rigorous. Here are the most notable qualities of a strong hypothesis:

Testability: Ensure the hypothesis allows you to work towards observable and testable results.
Brevity and objectivity: Present your hypothesis as a brief statement and avoid wordiness.
Clarity and Relevance: The hypothesis should reflect a clear idea of what we know and what we expect to find out about a phenomenon and address the significant knowledge gap relevant to a field of study.

Understanding Null and Alternative Hypotheses in Research

There are two types of hypotheses used commonly in research that aid statistical analyses. These are known as the null hypothesis and the alternative hypothesis . A null hypothesis is a statement assumed to be factual in the initial phase of the study.

For example, if a researcher is testing the efficacy of a new drug, then the null hypothesis will posit that the drug has no benefits compared to an inactive control or placebo . Suppose the data collected through a drug trial leads a researcher to reject the null hypothesis. In that case, it is considered to substantiate the alternative hypothesis in the above example, that the new drug provides benefits compared to the placebo.

Let’s take a closer look at the null hypothesis and alternative hypothesis with two more examples:

Null Hypothesis:

The rate of decline in the number of species in habitat X in the last year is the same as in the last 100 years when controlled for all factors except the recent wildfires.

In the next experiment, the researcher will experimentally reject this null hypothesis in order to confirm the following alternative hypothesis :

The rate of decline in the number of species in habitat X in the last year is different from the rate of decline in the last 100 years when controlled for all factors other than the recent wildfires.

In the pair of null and alternative hypotheses stated above, a statistical comparison of the rate of species decline over a century and the preceding year will help the research experimentally test the null hypothesis, helping to draw scientifically valid conclusions about two factors—wildfires and species decline.

We also recommend that researchers pay attention to contextual echoes and connections when writing research hypotheses. Research hypotheses are often closely linked to the introduction ² , such as the context of the study, and can similarly influence the reader’s judgment of the relevance and validity of the research hypothesis.

Seasoned experts, such as professionals at Elsevier Language Services, guide authors on how to best embed a hypothesis within an article so that it communicates relevance and credibility. Contact us if you want help in ensuring readers find your hypothesis robust and unbiased.

References

Hypotheses – The University Writing Center. (n.d.). https://writingcenter.tamu.edu/writing-speaking-guides/hypotheses
Shaping the research question and hypothesis. (n.d.). Students. https://students.unimelb.edu.au/academic-skills/graduate-research-services/writing-thesis-sections-part-2/shaping-the-research-question-and-hypothesis

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How To Write A Research Paper

How To Write A Hypothesis

How To Write a Hypothesis in a Research Paper | Steps & Examples

13 min read

Published on: Aug 5, 2021

Last updated on: Oct 28, 2024

How to write a hypothesis in a research paper

What is a Hypothesis?

A hypothesis is a tentative explanation or prediction that can be tested through scientific investigation.

It is like a roadmap that guides researchers in their quest for answers. By formulating a hypothesis, researchers make educated guesses about the relationship between variables or phenomena.

Think of a hypothesis as a detective's hunch. Just like a detective forms a theory about a crime based on evidence, a researcher develops a hypothesis based on existing knowledge and observations.

Now that we have a basic understanding of what a hypothesis is, let's delve into the process of writing one effectively.

Variables in Hypothesis

In hypotheses, variables play a crucial role as they represent the factors that are being studied and tested.

Let's explore two types of variables commonly found in hypotheses:

1. Independent Variable: This variable is manipulated or controlled by the researcher. It is the factor believed to have an effect on the dependent variable. Here's an example:

Hypothesis: "Increasing study time (independent variable) leads to improved test scores (dependent variable) in students."

In this hypothesis, the independent variable is the study time, which the researcher can manipulate to observe its impact on the test scores.

2. Dependent Variable: This variable is the outcome or response that is measured or observed as a result of the changes in the independent variable. Here's an example:

Hypothesis: "Exposure to sunlight (independent variable) affects plant growth (dependent variable)."

In this hypothesis, the dependent variable is plant growth, which is expected to be influenced by the independent variable, sunlight exposure. The researcher measures or observes the changes in plant growth based on the different levels of sunlight exposure.

Research Question vs Hypothesis

A research question is an inquiry that defines the focus and direction of a research study. A hypothesis, on the other hand, is a tentative statement that suggests a relationship between variables or predicts the outcome of a research study.

Hypothesis vs. Prediction

The difference between a hypothesis and a prediction is slight, but it's critical to understand.

Hypotheses are a great way to explain why something happens based on scientific methods.

A prediction is a statement that says something will happen based on what has been observed.

A hypothesis is a statement with variables. A prediction is a statement that says what will happen in the future.

Theory vs. Hypothesis

The theory and hypothesis have some differences between them.

A hypothesis is the explanation of a phenomenon that will be supported through scientific methods.
A theory is a well-substantiated and already-tested explanation backed by evidence.

To turn a hypothesis into a theory, you need to test it in different situations and with strong evidence. Theories can also be used to make predictions about something that is not understood. Once you have predictions, you can turn them into hypotheses that can be tested.

How to Develop a Hypothesis Step by Step?

Developing a hypothesis is an important step in scientific research, as it sets the foundation for designing experiments and testing theories.

Let's explore the step-by-step process of developing a hypothesis, using the example of studying the effects of exercise on sleep quality.

Step 1. Ask a Question

To begin, ask a specific question that focuses on the relationship between variables. In our example, the question could be: "Does regular exercise have a positive impact on sleep quality?"

Step 2. Do Background Research

Before formulating your hypothesis, conduct preliminary research to gather existing knowledge on the topic.

Review scientific studies, articles, and relevant literature to understand the current understanding of exercise and its potential effects on sleep quality. This research will provide a foundation for formulating your hypothesis.

Step 3. Develop Your Hypothesis

Based on your question and preliminary research, formulate a hypothesis that predicts the expected relationship between variables. In our example, the hypothesis could be:

"Regular exercise has a positive influence on sleep quality, resulting in improved sleep duration and reduced sleep disturbances."

Step 4. Refine Your Hypothesis

Refine your hypothesis by making it more specific and testable. Specify the variables involved and the anticipated outcomes in clear terms. For instance:

"Engaging in moderate-intensity aerobic exercise for at least 30 minutes, three times a week, will lead to an increase in total sleep time and a decrease in the frequency of sleep disruptions."

Step 5. Express Your Hypothesis in Three Forms

To ensure comprehensiveness, phrase your hypothesis in three different ways: as a simple statement, as a positive correlation, and as a negative correlation. This will cover different perspectives and potential outcomes.

Using our example:

Simple Statement: "Regular exercise positively affects sleep quality."
Positive Correlation: "As the frequency of regular exercise increases, sleep quality improves."
Negative Correlation: "A lack of regular exercise is associated with poorer sleep quality."

Step 6. Construct a Null Hypothesis

In addition to the main hypothesis, it is important to write a null hypothesis. The null hypothesis assumes that there is no significant relationship between the variables being studied.

The example below shows how to state the null hypothesis in a research paper:

By following these steps, you can develop a well-structured and testable hypothesis that serves as a guiding framework for your scientific research.

Types of Research Hypotheses with Examples

Hypotheses come in various forms, depending on the nature of the research and the relationship between variables.

Here are seven common types of hypotheses along with examples:

Simple Hypothesis: A straightforward statement about the expected relationship between variables.

Example: "Increasing fertilizer dosage will lead to higher crop yields."

Complex Hypothesis: A hypothesis that suggests a more intricate relationship between multiple variables.

Example: "The interaction of genetic factors and environmental stressors contributes to the development of certain mental disorders."

Directional Hypothesis: A hypothesis that predicts the specific direction of the relationship between variables.

Example: "As temperature decreases, the viscosity of the liquid will increase."

Non-Directional Hypothesis: A hypothesis that suggests a relationship between variables without specifying the direction.

Example: "There is a correlation between caffeine consumption and anxiety levels."

Null Hypothesis: A hypothesis that assumes no significant relationship between variables.

Example: "There is no difference in exam performance between students who study in silence and students who listen to music."

Alternative Hypothesis: A hypothesis that contradicts or offers an alternative explanation to the null hypothesis.

Example: "There is a significant difference in weight loss between individuals following a low-carb diet and those following a low-fat diet."

Associative Hypothesis: A hypothesis that suggests a relationship between variables without implying causality.

Example: "There is a correlation between exercise frequency and cardiovascular health."

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What Makes a Good Hypothesis? 5 Key Elements

Crafting a good hypothesis is essential for conducting effective scientific research. A well-formed hypothesis sets the stage for meaningful experiments.

Here are some key characteristics that make a hypothesis strong:

1. Testable and Specific

A good hypothesis should be testable through observation or experimentation. It should be formulated in a way that allows researchers to gather data and evidence to support or refute it.

When writing a research hypothesis, it is crucial to structure it in a manner that suggests clear ways to measure or observe the variables involved.

2. Grounded in Existing Knowledge

A strong hypothesis is built upon a foundation of existing knowledge and understanding of the topic. By connecting your hypothesis to previous findings, you ensure that your research contributes to the broader scientific knowledge.

This incorporation of existing knowledge aligns with the concept of research hypotheses, where hypotheses are framed based on the understanding of the subject from previous studies.

3. Falsifiable

A good hypothesis must be falsifiable, meaning that it can be proven false if it is indeed false. This principle is important because it allows for rigorous testing and prevents researchers from making claims that are impossible to verify or disprove.

This aligns with the idea of statistical hypothesis, where hypotheses need to be formulated in a way that allows statistical testing to determine their validity.

4. Clearly Defines Variables

A well-formulated hypothesis clearly identifies the independent and dependent variables involved in the research. It specifies the relationship between two variables and states what researchers expect to find during the study.

The clarity in defining variables is a crucial aspect of developing logical hypotheses.

5. Supported by Logic and Reasoning

A good hypothesis is logical and based on sound reasoning. It should be supported by evidence and a plausible rationale. The relationship between two variables proposed in the hypothesis should be grounded in a solid understanding of cause-and-effect relationships and theories.

A strong hypothesis, whether it is a research hypothesis, statistical hypothesis, or logical hypothesis, encompasses these key elements. By incorporating these elements you lay the groundwork for a robust and meaningful research study.

Hypothesis Examples

Here are a few more examples for you to look at and get a better understanding!

How to Write a Hypothesis in Research

Research Question: "Does exposure to violent video games increase aggressive behavior in adolescents?"

Hypothesis 1: "Adolescents who are exposed to violent video games will display higher levels of aggressive behavior compared to those who are not exposed."

Hypothesis 2: "There is a positive correlation between the amount of time spent playing violent video games and the level of aggressive behavior exhibited by adolescents."

How to Write a Hypothesis for a Lab Report:

Lab Experiment: Testing the effect of different fertilizers on plant growth.

Hypothesis 1: "Plants treated with fertilizer A will exhibit greater growth in terms of height and leaf count compared to plants treated with fertilizer B."

Hypothesis 2: "There is a significant difference in the growth rate of plants when exposed to different types of fertilizers."

How to Write a Hypothesis in a Report:

Report Topic: Investigating the impact of social media usage on self-esteem.

Hypothesis 1: "Individuals who spend more time on social media will report lower levels of self-esteem compared to those who spend less time on social media."

Hypothesis 2: "There is an inverse relationship between the frequency of social media use and self-esteem levels among individuals."

Example of Hypothesis in a Research Proposal:

Crafting hypotheses in a research proposal is pivotal for outlining the research aims and guiding the investigative process. Here's an example of a hypothesis within a research proposal:

Research Proposal Topic: Investigating the impact of social media usage on adolescents' self-esteem levels.

Hypothesis: "Adolescents who spend more time on social media platforms will have lower self-esteem levels compared to those who spend less time on social media."

How To Write a Hypothesis Psychology

Research Topic: Investigating the impact of mindfulness meditation on reducing symptoms of anxiety in college students.

Hypothesis 1: "College students who regularly practice mindfulness meditation will report lower levels of anxiety compared to those who do not engage in mindfulness practices."

Hypothesis 2: "There will be a significant decrease in anxiety scores among college students who undergo a structured mindfulness meditation program compared to a control group receiving no intervention."

How to Write a Hypothesis for a Research Paper:

Research Paper Topic: Examining the effect of mindfulness meditation on stress reduction.

Hypothesis 1: "Participating in regular mindfulness meditation practice will result in a significant decrease in perceived stress levels among participants."

Hypothesis 2: "There is a positive association between the frequency of mindfulness meditation practice and the reduction of stress levels in individuals."

How to Write a Hypothesis for Qualitative Research:

Qualitative Research Topic: Exploring the experiences of first-time mothers during the postpartum period.

Hypothesis 1: "First-time mothers will report feelings of increased anxiety and stress during the early weeks of the postpartum period."

Hypothesis 2: "There will be a common theme of adjustment challenges among first-time mothers in their narratives about the postpartum experience."

Good and Bad Hypothesis Example

Below are examples of good and bad hypotheses, along with their corresponding research question and hypothesis examples:

In conclusion, a well-crafted hypothesis sets the stage for designing experiments, collecting data, and drawing meaningful conclusions.

By following the steps of formulating a hypothesis, researchers can ensure that their investigations are grounded in solid reasoning. AI essay writing tools can be a great help in getting ideas.

However, If you need assistance with essay writing, consider our expert research paper writing service . Our team of experienced writers is dedicated to delivering high-quality, customized essays that meet your requirements and deadlines.

Don't hesitate to visit CollegeEssay.org and benefit from our professional essay writing service . Contact us today and say goodbye to your academic paper-writing worries.

Frequently Asked Questions

What are the 3 required parts of a hypothesis.

The three main parts of the hypothesis are:

Problem
Proposed solution
Result

What are 5 characteristics of a good hypothesis?

The main five characteristics of a good hypothesis are:

Clarity
Relevant to problem
Consistency
Specific
Testability

What should not be characteristic of a hypothesis?

Complexity should not be a good characteristic of a hypothesis.

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As a Digital Content Strategist, Nova Allison has eight years of experience in writing both technical and scientific content. With a focus on developing online content plans that engage audiences, Nova strives to write pieces that are not only informative but captivating as well.

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