frye and edidin experiment quizlet

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Question: You are performing an experiment similar to the one Frye and Edidin did to test membrane fluidity. But instead of fusing a mouse and human cell together, you decide to tag a small region of a mouse cell's membrane with fluorescent proteins. Based on what you know about the results of the mouse/human cell fusion experiment, what result do you predict you will

According to the Frye-Edidin experiment, when two cells fuse, their proteins disseminate throughout ...

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Biology ch 4 Practice Questions

General biology (bio 1100), eastern illinois university.

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Summary Questions for Chapter 4 – Membranes and Signalling

Explain the “Fluid Mosaic Model”. - Fluid- leppd by lyers - Mosaic - protein What hypothesis did the Frye-Edidin experiment support and why? - Hypothesis; membrane are fluid - They infused mouse & human cell then dyed half of the proteins (bottoms) later and found out that some of the dyed protein are on the top concluding proyrins have movement. Therefore , membrane are fluid What does it mean that membranes are asymmetrical? - Top of the membrane doesn’t look the same as bottom Explain the role of phospholipids in membranes. - Control membrane fluidity Why are phospholipids ideal at forming membranes? - They are both unsaturated & saturated maintain fluidity - Hydrophilic head that protects the hydrophobic tail In membranes how do saturated and unsaturated fatty acids affect fluidity? - Saturated - Hydrophobic tail is linear (more saturated) making the bilayer more dense (hard) - Unsaturated - Hydrophobic tail has double bonds, less structure makes the bilayer too fluid How does temperature affect the membranes? - Low temp = less fluid - High temp = more fluid What does desaturase do and how does it affect membranes? - Makes saturated fatty acid unsaturated to maintain fluidity - Active in low temp What does cholesterol do to membranes at high and low temperatures? - Sterol = maintain optional fluidity - Low temp = add to make space between the double bonds - High temp= Name the four main functions of membrane proteins? - Transport

Signal transduction

Attachment recognition What is the difference between peripheral and integral membrane proteins?

Peripheral a. On the surface of membrane b. Held together by non-counted bonds

Integral a. Embedded in the bilayer b. Can be transmembrane protein (both ends exposed) What allows integral membrane proteins to embed within the phospholipid bilayer?

Made up of non-polar amino acids 1 bilayer is non polar What are transmembrane proteins?

They are integral protein but the ends stick out of the by layer , ends are polar Why are most molecules restricted from moving across the cell membrane?

Hydrophobic nature of the bilayer restrictes free movement What is diffusion?

Movement of substance from high to low concentration

Doesn’t require free energy = spontaneous What are the similarities and differences between simple diffusion and facilitated diffusion?

S= both high ->low concentration , both are spontaneous

D= How is simple diffusion different from facilitated diffusion?

Substances go straight thru the phospho lipid by layer

Mostly small

-ive charged ions

Large or charged molecule that can go thru (water) will take time

Facilitated

Requires integral proteins

Channel 1 carrier

Large & +ve charged molecules Which types of molecules can cross cell membranes by simple diffusion?

Small uncharged molecule cuz it doesn’t disrupt the hydrophobic part of the bilayer

Secondary active transport uses it What is the difference between antiport and symport during secondary active transport?

antiport : driving ion moves in the opposite direction from solute “Solute always go low ->high making it spontaneous “Driving ions moves high->low”

Symport: driving ion moves in the same direction as solute What is the difference between exocytosis and endocytosis?

Exo: move large particle out of cell

End: moves large things into the cell “Both require energy” Proteins that are secreted from the cell via exocytosis were originally synthesized in what organelle?

RER What is the difference between pinocytosis and receptor mediated endocytosis?

Pinocytosis: visible brings: visible liquid ion

receptor mediated endocytosis: bring in solute that attached to receptor How is LDL which contains cholesterol taken up by cells?

Receptors LDL defective -> gene is mutated -> functions not right

LDL receptor binds to LDL particle & clear blood of LDL particle

Detect LDL particle will start to collect & accumulate Which cells in our bodies are involved in phagocytosis?

While blood cells engulf bacteria In cell communication, what is the difference between a control and target cell?

Control cell - creates or releases a signal

Target cell - processes signals in 3 steps

• Reception - receiving signal
• Transduction - releasing it to cell
• Multiple Choice

Course : General Biology (BIO 1100)

University : eastern illinois university.

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